Dextran component determined the separation properties of these media. Superdex 200 10 300 gl from ge healthcare life sciences. Superdex 75 10 300 gl is a prepacked gel filtration column for highresolution, semipreparative, and analytical separations of biomolecules. Ge healthcare size exclusion chromatography superdex 75. Quick information superdex 200 increase 5150 gl and. Superdex 75 10300 gl prepacked column for smallscale preparative purification g to mg or characterization of proteins in the molecular range 3000 to 70 000, like recombinant, tagged proteins. Superdex is the first choice for highresolution fractionation, short run times, and high recovery. Ge healthcare hiload 16600 superdex 200 pg hiload 1660.
Flow rate limits and recommendations superdex 75 increase 10300 gl note. Superdex 200 10300 gl chromatographic separation column from. Highsample load 500 l separation of a mixture containing seven. Superdex prep grade pg is produced by covalent bonding of dextran to highly crosslinked agarose. Ge healthcare hiload 16600 superdex 200 pg promotion. Superdex prep grade is a composite matr ix of dextran and highly crosslinked agarose. As a current student on this bumpy collegiate pathway, i stumbled upon course hero, where i can find study resources for nearly all my courses, get online help from tutors 247, and even share my old. The column is supplied with two fingertight connectors 116 male for connection to.
Superdex 200 hr 1030 instructions superdex 200 hr 1030 is a prepacked column for high performance gel filtration of proteins, dna fragments superdex increase and superose increase. For purification with larger sample volumes use hiload tm. A stepbystep method for the reconstitution of an abc. Hiload 1660 or 2660 superdex 200 prep grade dismantling tool, support screens, net rings. Elution volumes for globular proteins of various molecular weights on superdex 75 increase 10 300 blue, superdex 200 increase 10 300 green, and superose 6 increase 10 300 orange. Superdex 75 has a separation range for molecules with molecular weights between 3 000 and 70 000. Why the column pressure of superdex 75 10300 gl has. Superdex 75 10300 gl is a prepacked gel filtration column for highresolution, semipreparative, and analytical separations of biomolecules. Superose 6 hr 1030 superose 12 hr 1030 52176800 edition ak connecting the column to hplc systems columns prepacked with superose can be used with any hplc system if the pump can provide precise and accurate flow at relatively low backpressures. Rapid purity check and screening superdex 75 5150 gl, superdex 200 increase 5150 gl and superose 6 increase 5150 gl are short columns with small bed volumes that are suitable for rapid protein homogeneity analyses or purity checks. Filled with stationary phase compositions or resins, the prepacked options superdex 200 manual ge healthcare superdex 200 increase 10300 gl prepacked. Column lengths of 300 and 600 mm are also available for the 10 mm diameter columns. Superdex 200 hr 1030 instructions superdex 200 hr 1030 is a prepacked column for high performance gel filtration of proteins, dna fragments 10300 gl prepacked tricorn columns are designed for small scale preparative purification.
Ace2 and the 2019ncov rbdsd1 were incubated with 10 % wtwt hrv3c protease for 2 hours at room temperature. Superdex 200 increase 10 300 gl in lot release superdex 200 10 300 gl size exclusion chromatography sec columns are used as part of product lot release for several proteinbased drugs, to quantify aggregates andor protein fragments. Ge healthcare lifescience superdex 200 increase 10300 gl prepacked tricorn columns are designed for small scale preparative purification. Superdex 200 10 300 gl and and 5150 gl have been discontinued and are replaced by superdex 200 increase gl columns. Steep selectivity curves give gppd resolving power for peptides and proteins in the molecular weight range mr 10 000 600 000 superdex 200 pg. Superdex 200 10300 gl 1 17517501 superdex 75 10300 gl 1 17517401 superdex peptide 10300 gl 1 17517601 hiload 1660 superdex 200 pg 1 17106901. Instructions 56119099 ae gel filtration media superdex 30. Protein purification chromatography calibration of. Superdex 200 increase prepacked columns are designed for size exclusion chromatography sechigh resolution gel filtration in smallscale g to mg, preparative purification, as well as for characterisation and analysis of proteins with molecular weights mr from 10 000 to 600 000. Reverse the flow direction and pump 69 ml for superdex 75 5150 gl and superdex 200 5150 gl 80100 ml for superdex 75 10300 gl, superdex 200 10300 gl, superdex peptide 10300 gl of well degassed eluent buffer through the column at a flow rate of 0. Pdf legumain expression, activity and secretion are.
For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Superdex 200 10300 gl cytiva formerly ge healthcare. Ge life sciences 28990944 superdex 200 increase 10300 gl. Superdex 75, superdex 200 increase and superose 6 increase. Instructions 56119099 ae gel filtration media superdex. Gel filtration chromatography columns, superdex 200. Coupling multi angle light scattering to ion exchange. Highly suitable for the polishing step in a purification procedure. Size exclusion chromatography products cytiva formerly. Superdex 200 10300 gl and and 5150 gl have been discontinued and are replaced by superdex 200 increase gl columns. Feb 03, 2015 ordering information code no product 28990944 superdex 200 increase 10300 gl 28990945 superdex 200 increase 5150 gl 28990946 superdex 200 increase 3. A maximum of 40 mg protein was loaded on to a hiload 16600 20 superdex 200 pg ge healthcare for sizeexclusion chromatography and for buffer 21 exchange to 50 mm triscl ph7, 300 mm kcl, 15 mm mgcl2 for subsequent rna 22 measurements. After concentration by ultrafiltration the proteins were snap frozen in liquid.
Superdex 75 has a separation range for molecules with molecular weights between 3,000 and 70,000. Instruction 29163059 ae size exclusion chromatography. Fractions containing nanodiscs are pooled and concentrated to 10 mgml with an amicon centrifugal filter. Improved performance using new superdex 200 increase 10. Superdex 200 10300 gl chromatographic separation column. Elution volumes for globular proteins of various molecular weights on superdex 75 increase 10300 blue, superdex 200 increase 10300 green, and superose 6 increase 10300 orange. Size exclusion chromatography analysis of papaincleaved. New superdex 200 increase columns product description ge cat. Superdex 200 10300 gl ge healthcare, 17517501, column. Ordering information code no product 28990944 superdex 200 increase 10300 gl 28990945 superdex 200 increase 5150 gl 28990946 superdex 200 increase 3.
Superdex 200 10300 gl cytiva formerly ge healthcare life. Intended use superdex 75 increase 5150 gl an d superdex 75 increase 10300 gl columns are intended for research use only, and shall not be used in any clinical or in vitro procedures for diagnostic. Covalently circularized nanodiscs for studying membrane. Superdex peptide 10300 gl ge healthcare, 17517601, column l. Superdex 75 10300 gl from ge healthcare selectscience. Ge healthcare superdex 200 increase 10300 gl prepacked. Ge healthcare hiload 26600 superdex 200 pg hiload 2660. Superdex 75 5150 gl, superdex 200 increase 5150 gl and. Instruction 29027271 ah size exclusion chromatography columns. Separation of the monomer and dimer of a monoclonal antibody on superdex 200 increase 10300 gl. Cleaning gel filtration column which is the best cleaning.
Promo code superdex 200 increase 10300 gl suitable for both smallscale mg, preparative and highresolution analysis applications 28990944 45002570 45002570pr superdex 200 increase 5150 gl designed for rapid purity checking and quick screening of different. We dont know when or if this item will be back in stock. Superdex 200 10 300 gl is a prepacked gel filtration column for highresolution, semipreperative and analytical separations of biomolecules. These versatile columns offer rapid separations with high resolution for a variety of applications. The columns are prepacked glass columns for high performance gel filtration of proteins, peptides, dna fragments superdex 200 increase 5150 gl and superdex 200 increase 10 300 gl columns are intended for research use only, and shall not be used in any clinical or in vitro procedures for diagnostic purposes. Superdex 75 10300 gl ge healthcare, 17517401, column l. Superdex peptide 10300 gl ge healthcare, 17517601, column. Superose 6 hr 1030 superose 12 hr 1030 instructions. Size exclusion chromatography columns and media selection guide.
Superdex prepacked columns are designed for highperformance, laboratoryscale separations of proteins, peptides, and other biomolecules according to size. Superdex 200 has a separation range for molecules with molecular weights between 10 000 and 600 000. As shown in figures 4 a and b, the detection signal from the run with superdex 200 increase 3. Increased backpressure over the column andor loss of resolution. Intended use superdex 200 increase 5150 gl and superdex 200 increase 10300 gl columns are intended for research use only, and shall not be used in any clinical or. Instruction 29027271 ah size exclusion chromatography. For biomolecules in the 5650 kda molecular weight range. Cleaning gel filtration column which is the best cleaning solution. As protein drugs are getting increasingly complex at the same time as the. General description superdex peptide 10 300 gl is a prepacked tricorn highperformance column for highresolution gel filtration of peptides and other small biomolecules with molecular weights between 100 and 7000. Calibration of hiload 2660 superdex 200 prep grade. New superdex 200 increase columns fisher scientific. The superdex 200 increase 10 300 gl column is a versatile tool in your protein purification toolbox, perfectly suited for e. Scalability and high resolution 0 2 4 6 8 10 12 14 min mau 0 25 50 75 100 125 150 175 200 zenix, 3 mm srt, 5 mm srt10, 10 mm.
The column should be connected as described for fplc system via two unions which. Reverse the flow direction and pump 69 ml for superdex 75 5150 gl and superdex 200 5150 gl 80100 ml for superdex 75 10 300 gl, superdex 200 10 300 gl, superdex peptide 10 300 gl of well degassed eluent buffer through the column at a flow rate of 0. Safety for use and handling of the products in a safe way, refer to the safety data sheet. Hiload 16600 and 26600 superdex 30 prep grade, hiload 16600 and 26600 superdex 75 prep grade, and hiload 16600 and 26600 superdex 200 prep grade pg are prepacked xk columns designed for preparative gel filtration. Gel filtration chromatography columns, superdex 200 increase. Superdex 30 increase 10300 gl 1 add to cart 28989331 superdex 30 pg size exclusion chromatography media prepacked into hiload 16600 column. The whole fractionation range of superose 6 increase is not covered in this diagram. With their high physical and chemical stability, very high batchtobatch reproducibility, and regulatory support file backup, superdex 30 prep grade, superdex 75 prep grade and superdex 200 prep grade are ideal for all stages of an industrial scale operation from research and. Highresolution separations of proteins, peptides, and other biomolecules according to size.
Superdex 30 prep grade hiload 1660 and 2660 superdex 75 prep grade hiload 1660 and 2660 superdex 200 prep grade instructions 71502020 af high performance columns ge healthcare hiload introduction hiload 1660 and 2660 superdex 30 prep grade, superdex 75 prep grade, and superdex 200 prep grade are prepacked xk columns designed. Ge life sciences 28990944 superdex 200 increase 10 300 gl, versatile prepacked column currently unavailable. Most waterbased buffers can be considered to be similar to water, for example phosphate and tris buffers. Superdex 200 increase 10300 gl and superdex 200 increase 5150 gl columns, step 1 in table 1 data not shown. Analysis of fabcontaining flowthrough fabcontaining flowthrough step 2 in table 1 from purification on hitrap mabselect sure fig 2a was analyzed by sec on superdex 200 increase figs 2b and c. The columns are available with inner diameters of 5 and 10 mm, and in lengths from 20 to 200 mm. For biomolecules in the 5650 kda molecular weight range the enrich sec columns run at low backpressure, allowing for more flow rate flexibility, both in terms of sample viscosities and temperature conditions. Instruction 29163059 ae size exclusion chromatography columns. Nanodiscs are purified by gel filtration chromatography as in step 2. Two gel filtration calibration kits are available for protein. Use the enrich sec 650 10 x 300 column for completing size exclusion separations in less than 30 minutes at fast flow rates. Protein purification chromatography calibration of hiload 1660. The columns are prepacked glass columns for high performance gel filtration of proteins, peptides, dna fragments 200 bp and other biomolecules. Superdex 75 10300 gl and superdex 200 10300 gl are tricorn high performance columns.
Hiload 16600 and 26600 superdex 30 prep grade hiload 16. New agarose based size exclusion chromatography sec columns. Separation of test substances on hiload 26600 superdex 30 pg. Superdex 200 10300 gl and 5150 gl cytiva formerly ge. We are using superdex increase 10300 gl size exclusion column in our lab and recently column. Rapid purity check and screening superdex 75 increase 5150 gl, superdex 200 increase 5150 gl and superose 6 increase 5150 gl are short columns with small bed volumes that are suitable for rapid protein homogeneity analyses or purity checks. Superdex prepacked columns are designed for highperformance, laboratoryscale separations of. Superdex 30 prep grade medium is optimized for proteinspeptides below m r 10 000.
Why the column pressure of superdex 75 10300 gl has increased to 1mpa even at a flow rate of 0. Igg pdf structural characterization of vapb46 antitoxin from. The medium has a broad fractionation range that allows separation of a large variety of proteins m r 10 000 to 600 000 with an optimized resolution for the antibody molecular weight range m r 100 000 to. How to connect superdex 200 column for reverseflow. Sepaxssrt10 sec300 gives consistently good resolution at different flow rates and is able to be run at higher flows than the ge superdex 200. Two gel filtration calibration kits are available for protein molecular weight determination by gel filtration. The enrich sec columns run at low backpressure, allowing for more flow rate flexibility, both in terms of sample viscosities and temperature. Superdex gel filtration media are listed in appendix c, table 1. Intended use superdex 200 increase 5150 gl and superdex 200 increase 10 300 gl columns are intended for research use only, and shall not be used in any clinical or in vitro procedures for diagnostic purposes. Superdex 200 10300 gl from ge healthcare life sciences.
126 806 1204 897 500 907 1070 1496 1266 844 671 1220 454 1456 186 166 1508 1241 923 204 468 1337 221 392 137 371 890 343 107 351 960 152 1153